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KMID : 0368420090520030229
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Journal of Plant Biology
2009 Volume.52 No. 3 p.229 ~ p.243
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Rice OsOPRs: Transcriptional Profiling Responses to Diverse Environmental Stimuli and Biochemical Analysis of OsOPR1
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Jang Sung-Kuk
Cho Kyoung-Won
Shibato Junko
Han Ok-Soo
Iwahashi Hitoshi
Tamogami Shigeru
Zargar Sajad Majeed
Kubo Akihiro
Masuo Yoshinori
Agrawal Ganesh Kumar
Rakwal Randeep
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Abstract
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The 12-oxo-phytodienoic acid reductase (OPR) is a key enzyme in jasmonic acid (JA) biosynthesis. Previously, we reported the presence of 13 OPR isogenes (OsOPR1-13) in rice. OsOPRs phylogenetically belong to two subgroups, OPRI and OPRII. OsOPR13 is assigned to the second subgroup, which is involved in JA biosynthesis, while the others are found in the first subgroup. Here, we systematically investigated transcript levels of OsOPRs in various tissues and against diverse environmental stresses. Each gene was differentially involved in flower maturation, showing a tissue-specific response. OsOPR1, OsOPR2, and OsOPR13 were also active in responses to wounding, a fungal elicitor (chitosan), salt, UV-C irradiation, H2O2, and ozone exposure. In the case of JA-responsive OsOPRs (OsOPR1, OsOPR2, OsOPR6, OsOPR10, and OsOPR13), co-application of JA and SA suppressed jasmonate-induced transcript levels and delayed OsOPR10 expression. We also investigated the biochemical properties of OsOPR1 and found a flavin cofactor with optimal activity at pH?7.8 and values of 0.048 min?1 kcat and 8.33 μM Km for (9S,13R)-12-oxo-phytodienoic acid. Here, we discuss the role of OsOPRs in stress responses and floral development.
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KEYWORD
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Defense/stress response, Jasmonic acid (JA), Oryza sativa, 12-oxo-phytodienoic acid reductase (OPR), Oxylipin
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